Abstract: Presbycusis also known as Age-related hearing loss is a multifactorial genetic disease described as the degeneration of the auditory function by aging. This study aimed to gather all the genes associated with Presbycusis and identify the most important genes. Additionally, we determined the most critical biological function linked to this disorder. Materials and methods: The genes related to Presbycusis were systematically collected by literature review via PubMed advanced rearch. We then selected articles that showed a significant association between various genes and presbycusis (p-value<0.05). We utilized the GeneMANIA Cytoscape software and CytoHubba plugin within Cytoscape to explore the biological connections between these genes, create a visual gene network, identify crucial genes and also determine most important biological function. The gene network was created by GeneMANIA algorithm based on protein-protein interaction, genetic interaction, co-localization and pathway. Result: We found 78 genes related to Presbycusis. “Signal transduction in absence of ligand” with q-value 0.009 is the most significant biological function. The enrichment score is 28.31. The network revealed that Protein-Protein interactions among Presbycusis-related proteins is the most prevalent biological connection (78%). Additional the five top gene hubs according to the CytoHubba (MCC method) are: PTK2, CTH, DCLK1, GRM7 and TLR4. Conclusions This study shows that PTK2 gene is the most important gene in Presbycusis. Our study provides insight about the prioritization of the genes and biological function related to Presbycusis.
