مشخصات پژوهش

عنوان	Cellular and Humoral Immune Responses against AS03-Inactivated SARS CoV-2 Vaccine
نوع پژوهش	مقاله ارائه شده
کلیدواژه‌ها	inactivated vaccine, covid19, AS03, IFN-γ, IL-4
چکیده	Background: Several vaccines in the world are commercialized for COVID-19 infection. One of these vaccines is inactivated SARS-CoV-2 vaccine which is formulated in Alum adjuvant that is not capable of the induction of cellular immune responses. In the present study, inactivated SARS CoV-2 virus was formulated in AS03 adjuvant and the immunogenicity was compared with the Alum-based vaccine. Methods: Experimental 6-8-week-old BALB/c mice were immunized three times at three weeks intervals with AS03- and Alum-based vaccines along with PBS as a control group. Lymphocyte proliferation of spleen cells was performed by the BrdU method, IL-4 and IFN-γ cytokines were assessed on the spleen cell culture supernatant by quantitative ELISA kits. Specific total IgG and IgG1/IgG2a were assessed with an optimized indirect ELISA. Results: The results showed that the vaccine formulated in AS03 adjuvant led to a significant lymphocyte proliferation response versus Alum-based and PBS control group. Conclusion: In addition, the cytokines responses showed a Th1 platform in our vaccine formulation. Furthermore, IgG response showed an improvement versus the other experimental groups.
پژوهشگران	اکبر خراسانی (نفر سوم)، مهدی مهدوی (نفر چهارم)، فاطمه رودباری (نفر دوم)، مهلا عباسی (نفر اول)