مشخصات پژوهش

عنوان	Cloning and optimizing the expression of asparaginase enzyme from Bacillus subtilis
نوع پژوهش	مقاله ارائه شده
کلیدواژه‌ها	Cloning, Expression, Enzyme, Asparaginase
چکیده	INTRODUCTION L-Asparaginases (L-ASNase, EC 3.5.1.1) are enzymes that catalyze the hydrolysis of L- asparagine to L-aspartic acid and are found in various organisms from microorganisms to mammals. However, they are mainly expressed and produced by microorganisms. Microbial L-asparaginases have received ongoing attention because of their unique role in the treatment of acute lymphoblastic leukemia and because they inhibited acrylamide formation during food processing. MATERIALS AND METHODS The asparaginase enzyme gene was amplified from Bacillus bacteria and placed in the pet26 vector by heat shock method and transformed into BL21 bacteria and its expression was induced by EPTG. RESULTS AND DISCUSSION To confirm the methods of bacterial growth in two culture mediums containing the drug kanamycin and without the presence of this drug, it was observed that the transformed bacteria grew in the culture medium containing kanamycin and this method was confirmed by PCR and electrophoresis techniques. CONCLUSION Since the asparaginase enzyme was produced well in this part of the research, it can be used to advance therapeutic goals in other aspects of research.
پژوهشگران	علی طراوتی (نفر دوم)، فاطمه هاشمی (نفر اول)