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چکیده
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The use of nanomaterials as a carrier for enzyme immobilization has received attention in recent years, because of their large available surface; It offers higher enzyme-loading capacity, decreased mass transfer resistance, and increased mechanical resistance. The use of Magnetic Nanoparticles (MNPs) for immobilization is extensively studied. Chitosan is used as a surface coating agent for the synthesis of nanoparticles. Among its features are its non-toxicity, biodegradability, and biocompatibility, which has led to its extensive use in the food, cosmetic, pharmaceutical, and biotechnological industries. A magnetic nanoparticle coated with chitosan-PVA was synthesized in the first step by co-precipitation. For this purpose, polyvinyl alcohol (PVA) and chitosan were added to an acetate buffer with a pH of 5.4. In the next step, FeCl2, FeCl3, and then sodium hydroxide (NaOH) were used to create a magnetic state in nanoparticles. After that, the cross-linker epichlorohydrin (ECH) was added to the compounds in order to link chitosan to PVA on the nanoparticle surface. As the next step, it was placed in a 60°C for one hour and then washed and separated with a magnet. In spite of the polymer coatings formed on the surface of the magnetic iron nanoparticle, the particles showed good magnetic strength. By using an external magnet, it was well separated from the solution. Based on the results of the FTIR analysis, chitosan-PVA nanoparticles were found to be formed well. As a result, chitosan-PVA nanoparticles can be used for enzyme stabilization and drug delivery
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