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چکیده
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Polycyclic aromatic hydrocarbons (PAHs) are among the widespread environmental pollutants that are considered a serious threat in aquatic ecosystems. Among these compounds, benzoalphapyrene (BαP) is the primary form of five-ring PAHs, which is known to be genotoxic, mutagenic, and teratogenic for aquatic organisms. This study aims to evaluate and compare the bioinformatic gene profile in the liver of zebrafish exposed to 0.3 mg/L of BaP in order to introduce possible genetic markers to recognize the pathways of toxicity and predict this toxicity. The raw data of microarray expression was evaluated qualitatively and quantitatively. Then it was normalized using RMA algorithm and subjected to statistical analysis and comparison using t-test. The most genetic changes in the liver after exposure to BaP were related to the increased expression of CYP1A and CYP1b family genes and pro-oncogene junb, which were involved in the detoxification process and oxidative stress. Also, in the exposure group compared to the control group, the greatest decrease in expression was related to the expression of CYPC19, ER a and vtg1 genes, which play a role in sexual reproduction and the production of sex hormones. In general, the results showed that exposure of fish to BaP causes oxidative stress and stimulation of pro-oncogenes and increased inflammation in the liver tissue, and then the occurrence of reproductive and gender disorders in zebrafish, and the main expression changes of hepatotoxicity after exposure of fish to BaP.
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