مشخصات پژوهش

عنوان	Identification and determination of (Z)-11-hexadecenal in sex pheromone of Helicoverpa armigera by GC–MS and bacterial bioluminescence methods
نوع پژوهش	مقاله چاپ شده
کلیدواژه‌ها	(Z)-11-hexadecenal, Helicoverpa armigera, Pheromones, Bacterial bioluminescence, GC–MS
چکیده	Helicoverpa armigera (Lepidoptera: Noctuidae) is a widespread kind of pest in Iran and many countries around the world which are economically important. )Z(-11-hexadecenal has been identified as a major component of the female sex pheromone of H. armigra moths. In the present study, gas chromatography-mass spectrometry (GC–MS) was used for the identification and determination of (Z)-11-hexadecenal. (Z)-11-hexadecenal is a long-chain aliphatic aldehyde. It was also measured by bacterial bioluminescence assay based on Luciferase-FMNH2 complex, NADH, aldehyde and O2 as a sensitive and selective method. The detection limit of )Z(-11-hexadecenal was obtained 0.019 mg/L in the linear range of 0.05–4.5 mg/L by the bioluminescence assay and the GC–MS analysis amount was 1.5 mg/L with the linearity range of 5–50 mg/L. The relative standards deviation for three measurements was 1.92 and 5.7% by bioluminescence assay and GC–MS, respectively. The average amount of (Z)-11-hexadecenal in sex pheromone per moth in 2–3 days old virgin female with the bioluminescence assay and GC–MS were obtained 3.2 and 3.02 ng ml−1, respectively. Afterward, the amount of (Z(-11-hexadecenal was compared with 8–10 days old adult females by bacterial bioluminescence assay.
پژوهشگران	هدی عاصمی (نفر چهارم)، مجتبی محسنی (نفر سوم)، سماحه محمدخانی (نفر دوم)، محمد جواد چایچی (نفر اول)