11 بهمن 1401
اميد جزايري

امید جزایری

مرتبه علمی: استادیار
نشانی:
تحصیلات: دکترای تخصصی / ژنتیک
تلفن: 011-35302450
دانشکده: دانشکده علوم پایه

مشخصات پژوهش

عنوان Up-regulation of Arl4a gene expression by broccoli aqueous extract is associated with improved spermatogenesis in mouse testes
نوع پژوهش مقاله چاپ شده
کلیدواژه‌ها
Brassica, spermatogenesis, gene expression, mice, cadmium
مجله Biomédica
شناسه DOI doi: https://doi.org/10.7705/biomedica.5962
پژوهشگران امید جزایری (نفر اول) ، ستاره فرهمند عراقی (نفر دوم) ، طاهره السادات آقاجانزاده (نفر سوم) ، فرشته میر محمدرضایی (نفر چهارم)

چکیده

Introduction: Broccoli (Brassica oleracea) is well known for its properties as an anticancer, antioxidant, and scavenger of free radicals. However, its benefts in enhancing spermatogenesis have not been well established. Objective: To study broccoli aqueous extract effects on sperm factors and the expression of genes Catsper1, Catsper2, Arl4a, Sox5, and Sox9 in sperm factors in mice. Material and methods: Male mice were divided randomly into six groups: (1) Control; (2) cadmium (3 mg/kg of mouse body weight); (3) orally treated with 200 µl broccoli aqueous extract (1 g ml-1); (4) orally treated with 400 µl of broccoli aqueous extract; (5) orally treated with 200 broccoli aqueous extract plus cadmium, and (6) orally treated with 400 µl of broccoli aqueous extract plus cadmium. We analyzed the sperms factors and Catsper1, Catsper2, Arl4a, Sox5, and Sox9 gene expression. Results: An obvious improvement in sperm count and a slight enhancement in sperm motility were observed in mice treated with broccoli extract alone or with cadmium. Sperm viability was reduced by broccoli extract except for the 200 µl dose with cadmium, which signifcantly increased it. Interestingly, Arl4a gene expression increased in the 400 µl broccolitreated group. Likewise, the Arl4a mRNA level in mice treated with cadmium and 200 µl of broccoli extract was higher than in the cadmium-treated mice. Furthermore, broccoli extract enhanced the mRNA level of Catsper2 and Sox5 genes in mice treated with 200 µl and 400 µl broccoli extract plus cadmium compared with the group treated solely with cadmium. Conclusion: The higher sperm count in broccoli-treated mice opens the way for the development of pharmaceutical products for infertile men.