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Title
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Immunotoxicity of estrogen and nonylphenol on apoptosis and expression of ERs in goldfish macrophage: Opening new avenue for discovering the role of experimental model systems and sexes
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Type
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JournalPaper
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Keywords
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Estrogen receptors, Goldfish, Xenoestrogen, Apoptosis, Macrophages
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Abstract
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The expression of estrogen receptors (ERs) and their roles in important cell processes such as apoptosis in the macrophages exposed to estrogen/xenoestrogen have remained a complex secret. This study focused on the expression of estrogen receptors (ERs) and the stimulation of apoptosis in the macrophages from the two sexes of goldfish (Carassius auratus) exposed to 17-βestradiol (E2) and nonylphenol (NP) under in vivo and in vitro conditions. For the in vivo experiment, fish were exposed to NP (10−6 M and 10−7 M) and E2 (10−6 M) for 24 days. Then, the head kidney macrophages from the male and the female goldfish were isolated and assayed. For the in vitro experiments, the macrophages derived from the two sexes were cultured in L-15 medium and exposed to E2 (150 nM) and NP (10 nM and 150 nM) for 3 days. The results showed that the three isoforms of ERs (ERα, ERβ1, ERβ2) were expressed in the goldfish macrophages. After the exposure of macrophages to NP and E2, sex-specific increase of ERs expression and apoptosis were observed (P < 0.05). The expression of ERα after NP treatment showed the highest alteration, with the response being concentration-dependent. The most alteration of ERs expression were observed in the in vivo experiment. This study provides insight to understand how exposure of the goldfish macrophages to E2 and NP can up-regulate the transcript levels of estrogen receptor subtypes and stimulate apoptosis.
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Researchers
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Amirparviz Salati (Fifth Researcher), Abdolali Movahedinia (Fourth Researcher), Ebrahim Zabihi (Third Researcher), Rashid Alijani Ardeshir (Second Researcher), Sara Rastgar (First Researcher)
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