عنوان
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Taxonomic status and genetic differentiation of Hyrcanian Castanea based on noncoding chloroplast DNA sequences data
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نوع پژوهش
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مقاله چاپ شده
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کلیدواژهها
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Castaneasativa, DNAbarcoding, Morphologicaltraits, Haplotypediversity
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چکیده
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The taxonomy and phylogenetic relationships among Castanea species were investigated using sequence data from the chloroplast trnL-F and trnH-psbA intergenic spacer regions. Samples included Castanea specimens of uncertain taxonomic affinity that were collected in the Hyrcanian forest of northern Iran. The trnL-F data were more informative than trnH-psbA, having seven parsimonyinformative sites. A low level of haplotype diversity was detected within Hyrcanian samples and the whole species of the genus Castanea. In thetrnL-F dataset, Castanea sativa and Castanea mollissima have unique character states that differentiate them from other species of Castanea. The genus Castanea was recovered as a monophyletic with high to moderate support when inferred from combined trnH-psbA and trnL-F spacer data. Two main lineages received minimal support in the trnL-F analysis, whereas trnH-psbA could not distinguish different species of Castanea from each other. Finally, low levels of haplotype diversity was found within small remnant stands of Castanea in the Hyrcanian forest, indicatingthatgeneticerosionmayincreasetheextinctionrisk for these valuable trees.
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پژوهشگران
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حامد یوسف زاده (نفر اول)، نیکولاس پی تیپری (نفر چهارم)، فاطمه اکبرزاده روشن (نفر سوم)، اباصلت حسین زاده کلاگر (نفر دوم)
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