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چکیده
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VariationinlevelsofphenolicacidsandflavonoidsinScrophulariastriataBoiss.cellsculturedinbothshakeflaskandbioreactor invitrosystems,wasstudiedatdifferentgrowthphases.Fourphenolicacids(cinnamic,salicylic,coumaric,andcaffeicacid),one stilbenoid (resveratrol), and seven flavonoids (diosmin, rutin, kaempferol, catechin, myricetin, quercetin, and luteolin) were analyzedbyhigh-performanceliquidchromatographywithphotodiodearraydetection.Productionofphenolicsinthebioreactor was higher than in shake flasks. Catechin was the most abundant flavonoid in both culture systems, while quercetin, which was detected only in the bioreactor, was the lowest amount represented (32.82 μgg −1 DW). Resveratrol accumulation in bioreactor cultureswas59.84-foldhigherthanthatinshakeflasks.Moreover,hierarchicalclusteringanalysisbasedonPearson’scorrelation coefficient confirmed a positive correlation between the growth phase and some metabolites. The flavonoid accumulation increased with the cells’ physiological age in the bioreactor. Principal component analysis showed that the time course of induction of phenolic acids, flavonoids, and a stilbenoid (resveratrol) was significantly correlated. These findings highlight the capacity of S. striata for large-scale production of desired phenolics using a bioreactor system.
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