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چکیده
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One of the main sites of the magnetic fields influence on living cells is the cell cycle. The intensity of this influencehowever,variesdependingonthecelltypeandthedurationofthetreatment.Suspensionofcultured tobacco cells (Nicotiana tabacum cv. Barley 21) were synchronized via sucrose starvation at their stationary growth phase. The cells were then exposed to 0.2mT SMF up to 24h. The progression of different cell cycle phases was monitored through flow cytometry in a time course manner. Expression of cell cycle controlling genes and amounts of certain signaling molecules were measured as well. Exposure to SMF delayed G1.S transitionwhichwasaccompaniedbydecreaseofcyclin-dependentkinasesA(CDKA)andD-typecyclin,butan increaseintheadenylylcyclase(AC),transcriptionfactorE2F,retinoblastomaprotein(Rbp),andCDK-inhibitor protein 21 (p21) transcript accumulation. Exposure to SMF also increased the contents of nitric oxide (NO), hydrogenperoxide(H2O2),andsalicylicacid(SA),comparedtothecontrolgroup.Theresultssuggestasignaling pathway triggered by SMF starting from accumulation of NO and H2O2 followed by downstream events includingtheincreaseofcyclicnucleotidesandsubsequentdecreaseofbothCDKAandCycD
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