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چکیده
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Breast milk is the optimal food for babies, but the sucking infant may be exposed to drugs during maternal pharmacotherapy. It is important to know the extent of drug transfer into human breast milk in order to assess the likely ‘dose’ ingested by the infant and therefore quantify risk [1]. so we need a simple and efficient extraction method. Air assisted dispersive liquid-liquid microextraction is a sensitive, rapid and efficient method for the extraction of celecoxib as well as its determination in breast milk. In this method (AALLME), which is a new version of dispersive liquid–liquid microextraction (DLLME) no dispersive solvent is required and dispersion of extraction solvent lighter than water, is carried out by air bubbles[2]. Fine organic droplets were formed by sucking and injecting of the mixture of aqueous sample solution and extraction solvent with a conventional 2 mL glass syringe for several times in the handmade centrifuge tube with narrow neck which was specially designed for ease of withdrawing supernatant phase [3]. The influence of different parameters affecting including the type and volume of the extraction solvent, sample pH, ionic strength and number of extraction cycles were evaluated and optimized. Under the optimum conditions (type and volume of the extraction solvent, 1-undecanol, 50μL; sample pH =7; salt content of 6% NaCl w/v and number of extraction cycles = 4 ), extraction recovery of 91% with relative standard deviation less than10% were obtained for 3 replicated measurments which are satisfactorily consistent with predicted results and limit of quantification (LOQ) of 1 μg L-1 were obtained. The proposed method was successfully applied for preconcentration of the celecoxib drug in breast milk.
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