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چکیده
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Phenolic compounds are one of the most important groups that have presenting antioxidant capacity [1]. Also flavonoids are a group of polyphenolic compounds that exist widely in the plant kingdom. They have been suggested to possess many properties like antioxidant activity, ROS scavengers, defense against phytopathogens and UV-light protection, signal molecules in plant-microbe interactions, legume nodulation, male fertility2.Several studies report that these compounds show a preventive effect against chronic disease which related to excess of free radicals. Resveratrol which are a class of polyphenols compounds that have been distributed in various plants. These have been reported many pharmaceutical effects such as antiinflammatory, heart-healthy and anti-cancer properties. During the past decade, a considerable progress has been made to stimulate formation and accumulation of secondary metabolites using plant cell cultures. Bioreactors are important tools for industrial production of secondary metabolites in biotechnology. In this study, initially, is prepared of plant cell culture of S.striata and then be proceed in bioreactor. The optimal conditions provided for the growth in the bioreactor such as light, temperature, pH. Growth parameters are measured by dry weight, Fresh weight, survival rate and growth rate. Growth curve of S.striata showed in bioreactor 25 days periods. Total phenol, flavonoid, flavonol were quantified by spectro-photometry and resveratrol analysed by HPLC method. The results showed that maximum growth and production of resveratrol were in dark conditions, temp=25˚C, pH =4.8, aggregation speed=110-170 rpm and flow= 0.5-1.5 lpm for control of aeration. . The highest amount of resveratrol was in 25th day about 59.84 fold increase compared to the shake-flasks.Total flavonoids were 926 μg/g DW in 25th and total phenol were 5208.5 μg/g DW in 5th . The present study showed the optimized condition for cell suspension cultures of S. striata and ma
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