|
چکیده
|
Podophyllotoxin (PTOX) is a aryltetralin lignan and used for the preparation of the anticancer agents: etoposide, etopophos and teniposide which show effects against several types of neoplasms [1,2]. Linum album is a medicinal plant that is known due to have PTOX and related lignans [3]. In present research, we investigated PTOX accumulation in hairy roots of Linum album co-cultured with arbuscular mycorrhizalike fungus of Piriformospora indica (Strain: AVPI-1) in presence of methyl jasmonate (MeJA). In order to, fungal mycelia at ratio 2.5 % (v/v) and 0.01 mM MeJA were added to hairy roots culture on 12th day of their cultivation and PTOX accumulation was assayed at the determinated time-course (24, 48 and 72 hour after feeding) by HPLC. The results showed the co-cultured roots with fungus observed PTOX content in the hairy roots (138.7 μg g-1 DW), after 24 hours. Although the elicitation of the hairy roots with MeJA enhanced the PTOX content about 1.6-fold compared with co-cultured media but combined MeJA with co-cultures root and fungus increased PTOX accumulation about 222 μg g-1 DW. Furthermore, the secretion PTOX to media was increased in the co-cultured media and elicited co-culture media. Moreover, the PTOX content was observed 1.3 mg l-1, in the hairy root media after 72 h of co-culture with the fungus. But in the elicited co-culture media the amount of PTOX was tiny. It seems fungus over time, with increasing cell membrane damage, induced plant defense responses that led to the dramatic extracellular release of PTOX. This can be used as technology in industrial systems using bioreactors to mass production this important medicinal metabolite.
|