مشخصات پژوهش

عنوان	OPTIMAIZATION THE EXTRACTION SYSTEMS OF VITEXIN AS THE MAJOR BIOACTIVE FLAVONOID IN PROSOPIS FARCTA LEAVES, CALLUS AND TRANSGENIC HAIRY ROOT
نوع پژوهش	مقاله ارائه شده
کلیدواژه‌ها	Prosopis farcta, vitexin, HPLC
چکیده	The use of Prosopis farcta (Leguminosae, subfamily Mimosoideae) as a traditional medicine for treatment of some diseases and disorders was well known from ancient times in Iran and the plant is valuable for its pharmaceutical properties. During this study vitexin was isolated by HPLC method from Prosopis farcta by 3 different extraction methods in which parameters as the type, concentration and pH of the extracting solvents considered. Extraction is the first crucial step in preparation of active ingredients from plant materials. Optimizing the extraction process with high yield may be the result of a synergistic combination of fundamental factors as extraction technique and efficacy of solvent extraction. Among different solvent systems used, methanolwater (40%, containing acetic acid 0.5%) was found to be the best solvent for vitexin extraction [1- 3]. The amount of vitexin extracted from the leaves was 0.554 mg g-1 DW. Whereas, vitexin was detected neither in the callus nor hairy root. It is likely that callus and transgenic hairy roots need some degree of differentiation to produce more and complicated flavonoids such as vitexin before a special flavonoid product can be synthesized. The present work suggest an efficient method for estimation the greatest content of vitexin analyzed by HPLC technique and introduced Iranian P. farcta as a suitable source of vitexin with anti-tumor activity.
پژوهشگران	نجمه احمدیان چاشمی (نفر سوم)، محسن شریفی (نفر دوم)، سمیه ظفری (نفر اول)