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چکیده
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(CA1) region of the hippocampus. The purpose of this study was to evaluate the effect of early exercise on inflammatory parameters and apoptosis in the CA1 area of the hippocampus following cerebral I/R in adult male rats. Methods: Male Wistar rats were randomly divided into four groups: (Sham, Exercise [Exe], Ischemia [ISC], and Ischemia + Early Exercise [ISC+EE]). The ISC+EE group initiated forced treadmill training at 24 h after surgery. Ischemia was induced by occlusion of both common carotid arteries for 45 min 48 h after the last training session, apoptotic cells were detected by TUNEL. Hematoxylin & Eosin (H&E) and Cresyl violet staining were used to assess the damage of the CA1region of the hippocampus. Caspase-3, nuclear factor kappa-light-chainenhancer of activated B cells (NF-κB) and Tumor necrosis factor-alpha (TNF-a) were determined by Immunofluorescence. NF-κB, TNF-a, B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), Neuronal Nuclear (NeuN) were evaluated by western blotting. Motor function was evaluated by a horizontal ladder test. Results: The results showed that early exercise could decrease the number of apoptotic cells, decrease the expression of NF-κB, TNF-a, caspase-3, Bax and Bax/Bcl-2 ratio as well as increase Bcl-2 and NeuN (p < 0.05, n = 8). These data were consistent with improved motor function (p < 0.05, n = 8). Conclusion: This study showed that early exercise prevents lesions induced by ischemia in the CA1 region via inhibiting inflammation and apoptosis and supports functional recovery following IR.
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