20 بهمن 1401
سلمان احمدي اسب چين

سلمان احمدی اسب چین

مرتبه علمی: دانشیار
نشانی: بابلسر، دانشکده علوم پایه
تحصیلات: دکترای تخصصی / میکروبیولوژی
تلفن: --
دانشکده: دانشکده علوم پایه

مشخصات پژوهش

عنوان Moderately Halophilic Bacteria and Their Industrially Important Enzymes from the Ancient Ecosystem Badab-e Surt
نوع پژوهش مقاله چاپ شده
کلیدواژه‌ها
Badab-e Surt, extracellular enzymes, Gammaproteobacteria, lipase, moderately halophilic bacteria
مجله Industrial Biotechnology
شناسه DOI DOI: 10.1089/ind.2018.0031
پژوهشگران لیلا ستاری فقیهی (نفر اول) ، باقر سیدعلیپور (نفر دوم) ، سلمان احمدی اسب چین (نفر سوم) ، غلامحسین ریاضی (نفر چهارم)

چکیده

The stability of enzymes produced by halophilic bacteria under harsh conditions makes them appropriate candidates as biocatalysts. The present study focuses on hydrolysis enzymes production by halophilic bacteria from Badab-e Surt springs and investigates their lipolytic activity. First, 24 bacterial isolates were screened and selected based on different morphological and biochemical properties. Next, the production of extracellular enzymes, such as lipase, protease, amylase, and other hydrolyses were studied. Five isolates with the maximum lipolytic activity were then selected for 16S rRNA gene analysis. Phylogenetic analysis of isolates showed that they belong to the class of Gamma-proteobacteria, and were most similar to the genera Halomonas, Marinobacter, Vibrio, and Idiomarina. The study of optimum growth conditions for isolates indicated that all strains were classified as moderately halophilic bacteria. The lipase-specific activity of the strains S-3, S-10, S-11, S-14, and S-18 were 63.84, 98.04, 154.63, 65.35, and 71.98 Units/mg of protein, respectively. Enzyme activity of isolates proved that isolates could be attractive options for biotechnological applications under the enzymatic processes