11 بهمن 1401
اميد جزايري

امید جزایری

مرتبه علمی: استادیار
نشانی:
تحصیلات: دکترای تخصصی / ژنتیک
تلفن: 011-35302450
دانشکده: دانشکده علوم پایه

مشخصات پژوهش

عنوان Riboflavin Induces Different Defense Responses against Pyricularia oryzae in Improved and Traditional Rice (Oryza sativa L.) Cultivars
نوع پژوهش مقاله چاپ شده
کلیدواژه‌ها
Fajr cultivar, Peroxidase isoenzymes, Phenylalanine ammonia lyase, Polyphenol oxidase, Tarom Mahali cultivar
مجله journal of agricultural science and technology
شناسه DOI
پژوهشگران طاهره السادات آقاجانزاده (نفر اول) ، امید جزایری (نفر دوم)

چکیده

Riboflavin (vitamin B2) affects plant growth and development and participates in a variety of redox processes that affect plant defense responses. Two rice cultivars Fajr (improved) and Tarom Mahali (traditional) were foliar sprayed with increasing concentrations of riboflavin (0, 0.5, 1, 1.5 and 2 mM) and subsequently infected by Pyricularia oryzae. Then, leaves were collected at 0, 2, 4, 6 and 8 days after infection and activity of Peroxidase (POD), PolyPhenol Oxidase (PPO), and Phenylalanine Ammonia Lyase (PAL) were measured. Results revealed that lesion size and percentage of infected rice plants in Fajr was higher than Tarom Mahali. In addition, riboflavin-induced resistance was higher in Fajr than in Tarom Mahali due to higher activity of POD, PPO and PAL in Fajr than Tarom Mahali, especially upon exposure of plant to 2 mM riboflavin. The intensity of the bands of peroxidase isoenzymes with low molecular weight was enhanced by increasing concentrations of riboflavin in both rice cultivars, whileelevated riboflavin concentration caused the synthesis of three new isoenzymes in Fajr (g, h, i) and one (f) in Tarom Mahali cultivars. It can be concluded that Fajr is more sensitive to infection of P. oryzae than Tarom Mahali. In addition, the activity of POD, PPO, and PAL enhanced intensity of peroxidase isoenzymes bands. Also, the synthesis of new isoenzymes by riboflavin showed that riboflavin-induced resistance was more effective in Fajr than in Tarom Mahali.