2024 : 5 : 22
Shadmehr Mirdar Harijani

Shadmehr Mirdar Harijani

Academic rank: Professor
Education: PhD.
Faculty: Faculty of Physical Education and Sports Sciences
Address: Professor of Exercise Physiology, Dep. of Exercise physiology, Faculty of sports Sciences, University of Mazandaran P.O.Box 47415-388 Babolsar,Mazandaran Province, IRAN Tel: +98 +111+35302201 & 35302254 Fax: +98+ 111+35302201
Phone: 01135302254


Tobacco carcinogen-induced alterations in expression of cellular stress response genes in lung of Wistar rats and the effect of exercise training
4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, Cell stress, Pulmonary, Swimming training
Journal journal of basic and clinical pathophysiology
Researchers Maryam Khalesi ، Shadmehr Mirdar Harijani ، Ali Samadi


Abstract Objective: Present study investigated the effect of tobacco-specific carcinogen, nicotine-derived nitrosamine ketone (NNK), on the expression of cellular stress response genes in lung tissue of Wistar rats. Moreover, the effect of exercise training on alterations of these genes in exposed rats was investigated. Materials and Methods: A total of 30 healthy Wistar rats were obtained and divided into the following group: (1) Control (CON), (2) NNK exposure (NNK, once a week / 12.5 mg per kg body mass for 12 weeks), and NNK + swimming training (NNK+ST, received NNK exposure + 25-60 min of aerobic swimming training / 5 days per week for 12 weeks). The mRNA expression level of nuclear factor kappa-B (NF-κB), forkhead box protein O3 (FOXO3), and Sirtuin-1 (Sirt-1) were determined in lung tissue by the quantitative real-time PCR. Results: The NNK exposure resulted in a significant reduction in mRNA expression of the NF-κB and FOXO3 (P=0.0001 and P=0.005, respectively), as well as a significant increment in mRNA expression of Sirt-1 (P=0.001) comparing to the CON group. Moreover, the ST in rats under NNK treatment led to a significant reduction in mRNA expression of NF-κB (P=0.0001), but it did not have any significant effect on mRNA expression of FOXO3 and Sirt-1 (P>0.05) comparing to the NNK group. Conclusion: These findings indicate that NNK exposure can lead to adverse alterations in the expression of cellular stress response genes in the lung tissue of Wistar rats and exercise training may not have any favorable effect on the expression of these genes in rats under NNK treatment.