The pollution of water, as the most considerable mineral in human lives, is meeting its climax as a problematic issue. Among various methods of purification of water, the biological methods appear to bear more safety and to cost cheaper than other methods for their practicably use of plants, fungi, yeast, and bacteria to absorb azo dyes from water. Obviously, the purification of water is necessary for the continuation of distinctive aspects of life; for instance, farming, and drinking. This analysis delves into investigating Bacillus sp. that was isolated from Babolrood river, Babolsar, Mazandaran, Iran. Congruently, a thorough observation occurs on the ability of this Bacillus sp. in different parameters; the dye concentration (50-1000 mg/l), the examination of the active and the passive form of bacterial conditions (live and dead), the adsorption and the impacts of adsorption agent, kinetics (0-180 min), and the determination of isotherm type. Linearly, during this research usage of (SEM) and (FT-IR) were analyzed to determine the shape of the bacteria and the absorption graph before and after dye absorption. Thereupon, the features like the temperature (10-50 0C), the concentration of bacterial biomass, the age of bacteria, pH (1-13), the shaker, the different methods of demolishing bacterium were evaluated. Additionally, the binary and ternary of dyes absorption process was carried out and applied on the Red Congo, the Malachite Green, and the Methylene Blue; as a result, it was observed that the bacteria is able to absorb these dyes from the experimental environment.
