This paper proposes a novel single-source high step-up asymmetrical multi-level inverter (MLI) topology based on Marx generator configuration with voltage multiplying capability. The operating principles of the proposed converter (which is named Marxiplier) are completely different with the presented MLIs in the literature. Proposed converter uses a low voltage DC source with high frequency inverter (low voltage AC input) and charge a series of capacitors multiple times of input voltage. These capacitors which works as asymmetrical sources can generate a high-amplitude AC output voltage with high number of levels. Another important aspect of this converter is producing negative levels in the output voltage without using high-voltage full-bridge inverter and reducing the number of the components. MATLAB simulations and a laboratory test results of a 21-level prototype are employed in order to verify the proposed converter operation.