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Maryam Mohadjerani

Maryam Mohadjerani

Academic rank: Associate Professor
ORCID:
Education: PhD.
ScopusId:
Faculty: Science
Address: Department of Molecular and Cell Biology, Faculty of basic sciences . .University of Mazandaran, Babolsar, Mazandaran, Iran
Phone: 01135302455

Research

Title
ACETYLCHOLINESTERASE INHIBITORY ACTIVITY OF DOREMA AMMONIACUM GUM EXTRACTS AND MOLECULAR DOCKING STUDIES
Type
JournalPaper
Keywords
Dorema ammoniacum, Acetylcholinesterase inhibition, LC–MS, Docking study
Year
2020
Journal INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH
DOI
Researchers Mohsen Mazaheritehrani ، Rahman Hosseinzadeh ، Maryam Mohadjerani ، mahmood tajbakhsh ، Samad Nejad Ebrahimi

Abstract

Ammoniacum gum is traditionally used as an expectorant, stimulant, antispasmodic, catarrh, asthma, chronic bronchitis, and enlargement of liver and spleen in Medicine. The Soxhlet extraction of the oleo gum resin of D. ammoniacum was carried out with organic solvents including hexane, dichloromethane, chloroform, ethyl acetate, and methanol. The screening of the anti-alzheimer activity of the extracts was measured by acetylcholinesterase (AChE) inhibitory activity, and the hexane (IC50 = 32.34 μg/ml) and chloroform (IC50 = 43.5 μg/ml) extract performed highest activity. A profiling experiment of the chloroform extract conducted by LC-MS and literature resources suggested six potential biologically active compounds including doremone A (1), kopetdaghin E (2), dshamirone (3), kopetdaghin D (4), kopetdaghin C (5), and ammodoremine (6). To confirm the responsibility of these compounds for the observed AChE inhibitory activity, the molecular docking studies of these compounds were performed with AChE enzyme which indicated that these compounds show higher AChE inhibitory activity than galantamine as positive control. The molecular docking study was carried out using Glide.