Magnetic materials due to easily separation from reaction media using an external magnetic field received increased attention in the industry. On the other hand, nanomaterials are innovative platforms which present high surface-to-volume ratio allowing low mass transfer limitations. A magnetic hybrid nanostructured material consisting of iron-oxide nanoparticles embedded in carboxymethyl cellulose (CMC) matrix was synthesized in this study. This nanocomposite was synthesized from the inorganic salts of Fe (II) and Fe (III) by coprecipitation method and then modification with carboxymethyl cellulose. To synthesis nanocomposite, 0.2 g iron oxide nanoparticle and 0.1 g carboxymethyl cellulose (CMC) dissolved in 10 mL acetic acid (5%) containing 400 mM of N-Hydroxysuccinimide (NHS) and 20 mM of 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as the cross-linker. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analyses indicated that CMC was successfully coated on the surfaces of magnetic nanoparticles. The prepared magnetic carboxymethyl cellulose nanoparticle was stable in solutions with different pH and easily collected with the aid of an external magnet. Overall, this magnetic carboxymethyl cellulose nanocomposite could be utilized as an appropriate and cost-effective matrix for enzyme immobilization.
