Abstract In recent years, there has been growing attention to fabricate electrospun naofibrous composites based on natural and synthetic biopolymers via commonly used technique of electrospinnig for wound dressing application. This strategy is focused on the wound healing acceleration by well-designed dressing that mimic the structure of natural extracellular matrix coupled with incorporation of polymeric biomaterials such as chitosan which is capable to promote the healing process. In current research, electrospun PVA/Chitosan/Starch nanofibrous mat were fabricated by electrospinning method. Then obtained nanofibrous mats were cross-linked to optimize the biodegradation rate followed by characterization and evaluation of their properties as wound dressings. SEM and AFM showed that bead-free electrospun mats were formed. The chemical structures of the mats were analyzed by FTIR. The suitable water absorption, porosity and water vapor transmission rate of fabricated mats revealed their capability in providing proper moist environment for wound area, appropriate wound breathing and also efficient wound exudates handling. Mechanical properties of the mats confirm the potential of obtained mats to protect wound during the healing time. Antibacterial analysis exhibited antibacterial activity of nanofibrous mats against both gram negative and gram positive bacteria. Besides, MTT assay demonstrated appropriate cytocompatibility and cell viability of the developed nanofibrous mats which were also verified with in vitro wound healing analysis using scratch assay. Finally in vivo assay confirmed that developed mats could properly accelerate the healing of fullthickness wounds, revealing the significant potential of the developed mats in wound dressing application.
