Zinc and iron as two important essential minerals for testis functions are controlled by Zinc transporters. It has been reported that zinc and its transporters are affected by nutrition and training. The main goals of the current experimentation were to the influences of training in combination with extraction of Ajwain seeds on rat testicular zinc and zinc transporters. Forty male rats were randomly assigned into four groups. Rats were orally received an aqueous Ajwain seed extraction (200 mg kg-1 ) and the saline groups were treated in the same manner. Results showed that gene expression of Znt5 had meaningfully changed in the ST group in comparison with SC (p = 0.004) and AT (p = 0.001) groups. Expression of this gene had meaningfully alteration in the AT group in comparison with ST (p = 0.036) and AC (p = 0.001) groups. Gene expression of Znt8 was also significantly increased in AT group compared to AC group (P = 0.010). Expression of Znt9 was also significantly increased in AT group in comparison with AC (P = 0.008) and ST (P = 0.026) groups. Expression of the other genes (Znt6, Zip7, Zip8, and Zip14) and also the content of Zn, Fe and glycogen did not show significant differences. The concurrent implementation of training and supplementation with the extract of Ajwain seed significantly modulated the expression levels of certain zinc transporters. These discoveries can offer new understandings into the underlying mechanisms of the effects of exercise and nutrition on testicular tissue.
