سید ناصر عزیزی

Today, the use of antibiotics for the prevention, control and treatment of poultry indusry is unavoidable and on the other hand requiring a modern approach that providing high sensitivity and specifcity, convenient and rapid method to detection antibiotics. Therefore, in this sudy, for the frs time, a wide range of antibiotics used in poultry indusry were sudied and monitored based on liquid chromatography techniques. Therefore, two liquid chromatography-mass spectrometers were used to prepare the samples with acetic acid and acetonitrile and z-sep+ compounds and with internal sandards of antibiotic groups of sulfonamides, quinolones and tetracycline and comprised the results with conventional common method. Because of using of polymeric interface, absorption a large number of antibiotics was possible, so a wide range of antibiotics could be measured without any setting back in satic phase efciency. At the preparation, the amount of sample used is lower than that of classical methods and the preparation seps get shorter. Steady isotope of internal sandard caused controls in the extraction, purifcation, injection and calculations of possible errors such as ion subsidence, injection error, percent recovery of drug and retention time correction and also the possibility to inject high volumes and increase the sensitivity in this method was improved. Therefore, exploitation two liquid chromatography devices, one for sample preparation provides big advantages, which alone lacks this feature. Meanwhile, simultaneously automate method reduces analysis time, tes coss and increases accuracy compared to classical method and the sample preparation gets very convenient and allows the ability to search for antibiotics in the produced green chicken.