Continuous exposure to electromagnetic radiation emitted from Wi-Fi equipment is an important environmental health concern in today's world. It is also important that residents who live close to Wi-Fi equipment may have more health concern compared with those keep away from such radiation. Some recent studies reported that exposure to Wi-Fi electromagnetic radiation may induced oxidative stress and decreased the levels of antioxidants in various tissue. Although the possibility of electromagnetic radiation exposure in adverse health effects have been proposed but additional well targeted research will be valuable to declare the remaining uncertainties. The aim of this study was to investigate the impact of radiation emitted from Wi-Fi equipment on antioxidant status. For this purpose, the total antioxidant capacity of plasma were determined in rat exposed to Wi-Fi equipment. Twelve male rats were selected for the study, which randomly divided into two equal groups, control (n=6) and exposure (n=6). On the 60th day of the study, the rats were anesthetized and blood samples were placed into EDTA tubes. Plasma samples were used to assay total antioxidant capacity by FRAP assay. Mean total antioxidant capacity in the Wi-Fi expose rat were significantly lower than control rat (949.76±89.73 U/mL vs. 1142.2±127.16 U/mL, P = 0.016). In conclusion, exposures to radiation emitted from Wi-Fi equipment were accompanied by decreased total antioxidant capacity in rats. Thus, oxidative stress may underlying cause in pathophysiology of electromagnetic radiation-induced health damages.