Background and Aim : In present times, there is widespread use of electromagnetic radiation-emitting devices particularly wireless in industrial, scientific, medical, military, and domestic applications, with potential leakage of such radiation into the environment. Several studies have suggested that it is possible electromagnetic radiation from Wi-Fi equipment enhance production of reactive oxygen species (ROS), including superoxide anion, hydrogen peroxide, and hydroxyl radicals. Catalase (CAT) is a member of the enzymatic antioxidant system in human body that neutralize excessive ROS produced in oxidative conditions. The aim of the present study was to investigate the possible effects of electromagnetic radiation from Wi-Fi equipment on CAT level in rat plasma. Methods : For this purpose, two group of rat involved in this study, one control group and other group exposed to radiation emitted from Wi-Fi equipment. The blood samples were taken from all the rats and isolated plasma was used to measure the catalase activity. Results : Catalase level of rat plasma was significantly decreased in Wi-Fi exposed rats (17.77±2.93Vs 28.49±4.49, P=0.005). According to our results, the antioxidative defense system such as catalase activity were attenuated in the blood of rat exposed to Wi-Fi radiation. Conclusion : In conclusion, exposure to radiofrequency radiation emitted from Wi-Fi may cause systemic oxidative damages by creating free radicals, which consequently inhibit catalase activity.