Laccase has a lot of importance in biotechnology and many industrial applications.Immobilized enzyme present enhanced activity and storage time, pH and thermal stability compared with free enzyme. In this study, Laccase enzyme immobilized onto the thiolated chitosan nanoparticle. The thiolated chitosan nanoparticle characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). Chitosan nanoparticle was synthesized using sodium tripolyphosphate (TPP) as a polymerizing agent. Thiolated chitosan nanoparticle was prepared by adding thioglycolic acid (TGA). Finally, to performing immobilization, laccase enzyme (0.025 g/ml) loaded onto thiolated chitosan nanoparticle in the solution with pH of 5.5 by 24 hours incubation time at room temperature. Under these optimum circumstances, the efficiency of laccase immobilization was on the range of 42-48%. Based on our results, thiolated chitosan nanoparticle can be used as an ideal support for laccase immobilization in the biotechnological application.
