Introduction & Objectives: Wireless internet is extensively used in homes, work and public places such as schools, hospitals, libraries and cafeterias. Wireless access points emit electromagnetic fields which may have adverse effects on health. There are limited studies which reported the effects of wireless emitted electromagnetic fields radiation on testis tissue in term of anti-oxidative defense potential. The aim of this study was to investigate superoxide dismutase (SOD) activity after continuous exposure to Wi-Fi equipment. Materials & Methods: For this purpose, 12 male rat were selected and equally divided in two groups; one group exposed to radiation from Wi-Fi equipment for 10 weeks and other selected as control. After completing the exposure period, the animals were anaesthetized and testis was achieved for further analysis. Assays of superoxide dismutase were carried out spectrophotometrically by the riboflavin-methionine method of Giannopolitis and Ries. Results: Analysis of testis homogenate indicated that SOD activity in Wi-Fi exposed group was significantly increased compared with control group (290.87±19.95 Vs 238.85±15.02 U/g tissue, P=0.006). Conclusion: These results indicate that exposure to Wi-Fi equipment lead to change in some key enzymes of oxidative defense system. It is proposed that further studies are required to reveal the exact mechanisms of effect of Wi-Fi radiation and examining the health threating effects of such radiation with different frequencies and longer periods.