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Title
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A Genosensor for Point Mutation Detection of P53 Gene PCR Product Using Magnetic Particles
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Type
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JournalPaper
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Keywords
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Magnetic beads; ELISA; Sandwich hybridization; Microfluidic platform; PCR products
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Abstract
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The development of an electrochemical genosensor involving DNA biotinylated capture probe immobilized on streptavidin coated paramagnetic beads and microfluidic based platform for the detection of P53 gene PCR product is reported. The novelty of this work is the combination of a sensitive electrochemical platform and a proper microfluidic system with a simple and effective enzyme signal amplification technology, ELISA, for detection of target DNA sequence and single nucleotide mutation in p53 tumor suppressor gene sequence. The biosensor has been applied to detect the PCR amplified samples and the results shows that it can discriminate successfully perfect matched DNA from mutant form.
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Researchers
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Jahan Bakhsh Raoof (Second Researcher), Marco Mascini (Not In First Six Researchers), Ilaria Palchetti (Not In First Six Researchers), Seyed Mahdi Golabi (Fifth Researcher), Simin Sharifi (Fourth Researcher), Mohammad Saeid Hejazi (Third Researcher), Ezat Hamidi-Asl (First Researcher)
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