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Title
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Effect of NaCl on antioxidant enzymes and protein profile in halophyte Aeluropus littoralis leaves
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Type
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JournalPaper
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Keywords
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Phenylalanine ammonia-lyase, Peroxidase, Development, Leaf, Salt stress
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Abstract
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The effect of increasing NaCl concentration on soluble protein content, electrophoretic profile of total protein and enzyme activity along leaves with different ages in Aeluropus littoralis was investigated. We compared protein content of selected leaves in all treatments. Analysis revealed that salinity induced changes in protein pattern in leaf 7 and 10 in comparison with leaf 4. Salinity caused proteins induction in leaf 4 unlike two others. Salt also decreased total soluble protein in leaf 10 which treated by 400 mM of NaCl. Activity of Peroxidase (POD), Phenylalanine ammonia-lyase (PAL) and Polyphenol oxidase (PPO) increased gradually up to NaCl concentrations of 400 mM. The highest enzyme activity was observed when plants were treated with 400 mM of NaCl. Increased PAL and POD activities in leaf 10 were positively correlated with increasing NaCl concentrations. Polyphenol oxidase showed differential activities in response to salt and types of leaves. A significant increase was detected in leaves 4, 7 and 10 under salt. Leaf 4 at 400 mM of NaCl had the greatest activity among the three leaves. These results suggested a correlation between the activation of above enzymes, protein changes and leaf age during salt treatments.
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Researchers
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ُSaeed Irian (Not In First Six Researchers), Sedigheh Kelij (Fifth Researcher), Masood Shokri (Fourth Researcher), Ghorbamali Nematzadeh (Third Researcher), Ahmad Majd (Second Researcher), Leila Haghighi (First Researcher)
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