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Abstract
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Recently, biomarkers have been used for diagnosis and prevention of several types of malignant or benign tumors such as the prostate. One of these markers is the LMTK2 gene that codes a transmembrane serine/tyrosine kinase that plays role in membrane transferring. LMTK2 may interact with myosin IV and this protein has been identified to regulate prostate specific antigen (PSA) and vascular endothelial growth factor (VEGF) which are related to tumorigenesis. Also, downregulation of LMTK2 might contribute to prostate cancer formation. To investigate the correlation of rs7791463 which is located on intron 9 of LMTK2 and benign prostatic hyperplasia (BPH), the Boiling DNA extraction and the PCR-RFLP methods have been used for the blood sample of 45 patients and 50 normal controls participants. The PCR-product has been 257bp which has been cut by NcoI restriction enzyme in G allele. There could be 3 types of bonds with 112bp, 145bp, and 257bp that for GG the first and 2nd bond, for AG all of 3 bonds, and for AA just the 3rd bond have been visualized on gel agarose from PCR-product with 257bp length. Statistical analysis revealed that the distribution of genotypes for 87851G>A transition does not show Significant different (P = 0.55) in case and control groups. In conclusion, the present study suggests that 87851G>A transition is not associated with BPH, but the other study could show a possible effect for AA genotype.
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