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Title
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Curcumin-loaded chitosan-alginate-STPP nanoparticles ameliorate memory deficits and reduce glial activation in pentylenetetrazol-induced kindling model of epilepsy
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Type
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JournalPaper
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Keywords
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Curcumin nanoparticles; Memory improvement; Anticonvulsant; Glial activation; Pentylenetetrazol
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Abstract
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Despite several beneficial effects of curcumin, its medical application has been hampered due to low water solubility. To improve the aqueous solubility of curcumin, it has been loaded on chitosan (CS)-alginate (ALG) - sodium tripolyphosphate (STPP) nanoparticles (NPs). Then, the effect of curcumin NPs on memory improvement and glial activation was investigated in pentylenetetrazol (PTZ)-induced kindling model. Male NMRI mice have received the daily injection of curcumin NPs at dose of 12.5 or 25 mg/kg. All interventions were injected intraperitoneally (i.p), 10 days before PTZ administration and the injections were continued until 1 h before each PTZ injection. Spatial learning and memory was evaluated using Morris water maze test after the 7th PTZ injection. Animals have received 10 injections of PTZ and then, brain tissues were removed for histological evaluation. Nissl staining was used to determine the level of cell death in hippocampus and immunostaining method was performed against NeuN and GFAP/Iba1 for assessment of neuronal density and glial activation respectively. Behavioral results showed that curcumin NPs exhibit anticonvulsant activity and prevent cognitive impairment in fully kindled animals. The level of cell death and glial activation reduced in animals which have received curcumin NPs compared to those received free curcumin. To conclude, these findings suggest that curcumin NPs effectively ameliorate memory impairment and attenuate the level of activated glial cells in a mice model of chronic epilepsy.
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Researchers
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Anahita Ebrahimpour (Not In First Six Researchers), Ali Akbar Moghadamnia (Not In First Six Researchers), Fatemeh Ahmadi (Not In First Six Researchers), Shahram Ghasemi (Not In First Six Researchers), Mohsen Khalili-Fomeshi (Fifth Researcher), Atefeh Akbari (Fourth Researcher), Maryam Ghasemi-Kasman (Third Researcher), Diana Anissian (Second Researcher), Mona Hashemian (First Researcher)
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