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Title
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1-Ethyl-3-methylimidazolium Ethylsulfate/Copper(II) as Catalyst for Lucigenin Chemiluminescence and its Application for Glucose Detection
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Type
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JournalPaper
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Keywords
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1-ethyl-3-methylimidazolium ethylsulfate, lucigenin chemiluminescence, H2O2, glucose, realworld sample
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Abstract
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The present study reports the effect of an ionic liquid (IL), 1-ethyl-3-methylimidazolium ethylsulfate ([Emim][EtSO4]), on the Cu2+-catalyzed lucigenin/H2O2 chemiluminescence system in the range of pH 6.5‒10. At pH 8.0, the greatest enhancement on light emission is observed, related to a strong interaction between Cu2+ and the imidazolium ring at this pH. Furthermore, the formation of IL microdomains in the aqueous solution enhances in solubility of N-methylacridone, an insoluble emitter that can be very effective in lucigenin chemiluminescence (Luci-CL). The mentioned findings encouraged us to suggest a novel and simple method for the determination of glucose with the detection limit of 7 μM at pH 8.0 based on the detection of H2O2 liberated in enzymatic reaction between glucose oxidase and glucose by the [Emim][EtSO4]/Cu2+-catalyzed Luci-CL system. Also, the developed method was successfully applied to the determination of glucose in real plasma and urine samples of diabetic patients and validated against colorimetric spectroscopy method.
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Researchers
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Mohammad Javad Chaichi (Third Researcher), Reza Akhoondi (Second Researcher), seyedeh olia Alijanpour (First Researcher)
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