|
Abstract
|
Linum album has been shown to accumulate some lignans with antiviral and anticancer properties such as podophyllotoxin (PTOX), 6-methoxy podophyllotoxin (MPTOX) and lariciresinol, which were originally isolated from an endangered species Podophyllum [1]. Biotechnological methods and using biotic and abiotic elicitors are being developmentally studied for their application as alternative ways of obtaining lignans as valuable metabolites [2]. Among biotic elicitors, fungal elicitors such as Piriformospora indica as arbuscular mycorrhiza-like fungi have resulted in significant enhancement in the production of phytochemicals in plant tissue cultures [3]. In this study, we perused the metabolite responses of L. album hairy roots to elicitor activity of different concentration of P. indica cell wall on lignans content. Hairy root cultures were obtained after genetic transformation of plant tissue with Agrobacterium rhizogenes strain LBA9402 [4]. Fungal Cell wall extraction was done upon De Pietro et al. (2009) [5]. In continue, 10 days hairy roots were supplemented by 0, 0.5, 1, 2.5 and 5% (v/v) of fungal cell wall filtrated (CWF) and growth and lignans accumulation were analyzed after 5 days of elicitation. Results showed that P. indica CWF with ratio 1% (v/v) induced the highest amount of PTOX and lariciresinolin the hairy roots 135.1 and 128.8 μg g-1 dry weight (DW) respectively. Although hairy root growth was decreased with the increase of fungal concentration in the media butthe greatest amount of MPTOX was observed in 2.5 % (v/v) of CWF (19 mg g -1 DW). As results described here, CWF can be considered for accumulation of lignans in the hairy roots. Also, our results mention potential on-demand metabolic engineering of lignan biosynthesis using differential fungal elicitors.
|