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Abstract
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An effective, sensitive, relatively-fast and cost-effective method was developed to determine four types of selected chlorophenols (CPs) including 4-cholorophenol, 2,4-dicholorophenol, 2,5-dicholorophenol and 2,4,6-tricholorophenol in several kinds of honey, black tea and bovine milk samples through applying Cellulose Nano- Fiber (CNF)-ImSalophen@Fe3O4. CNF-ImSalophen@Fe3O4 has been synthesized using a very simple, easy, cost-effective, efficient and fast method. First, a magnetic CNF was used as the substrate. Next, imidazole was created on the surface of the magnetic CNF. Thereafter, the Cl-Salophen Schiff base was accommodated on the prepared magnetic CNF-Im. The prepared sorbent was used for the extraction of selected analytes. The extracted analytes were desorbed via organic solvent and analyzed through high-performance liquid chromatographyultraviolet detection (HPLC-UV). The strong interactions between the sorbent and the selected analytes (intermolecular hydrogen bonding, and π-π interactions) made this sorbent capable of high extraction performance and capacity. Under the optimized condition, the method linearity was in the range of 0.2–500 μg/L for the selected analytes in water. The calibration curves showed a determination of coefficient higher than 0.9986. The limits of detection and limits of quantification were calculated to be between 0.05–0.14 μg/L and 0.16–0.46 μg/L, respectively. In order to calculate the method precision, the inter-day and intra-day relative standard deviations were determined to be between 3.2 and 4.7 % in three selected concentrations. Furthermore, the enrichment factors were found to be 36, 31, 33 and 37 for 4-cholorophenol, 2,4-dicholorophenol, 2,5-dicholorophenol and 2,4,6-tricholorophenol, respectively. Moreover, the calculated absolute recoveries were between 62 and 74 %. The proposed method was also employed for the analysis of various real samples such as honey, bovine milk and black tea. The obtained recoveries indicate
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