Introduction: Previous studies have confirmed the sidelong effects of Doxorubicin (DOX) on healthy tissues, but pretreatment effect of regular Aerobic exercise in restrain of hepatotoxicity induced by doxorubicin is not clear. The aim of this study was to investigate the pretreatment effect of regular aerobic exercise on hepatic oxidative stress induced with doxorubicin of Wistar rats in different age groups. Methods: In the experimental study, Forty-eight male wistar rats divided into three groups of age (three and 29 months) were again randomly separated into subgroups: control+DOX (20 mg/kg, n=8), and Training+DOX (T+DOX20mg/kg, n=8) Training+ Saline (T+S) groups. The training protocol included treadmill running progressively between 25 to 54 min/day and 15 to 20m/min, 5 days/week for six weeks. DOX and saline injection was performed 24 h after the last exercise session, and tissue collection was done 24 h after the injections. Results: The results showed that the DOX-induced did not significant effect on HSP70 of liver tissue of rats in different ages (young, middle-aged and elderly) (P=0.272). DOX-induced had a significant effect on MDA (P=0.002), PC (P=0.002) and GPx (P=0.001) of liver tissue of rats in different ages (young, middle-aged and elderly). Pre-treatment with aerobic exercise had a significant effect on changes of HSP70 (P=0.001), MDA (P=0.001), PC (P=0.001) and GPx (P=0.001) in DOX-induced liver in different ages rats (young, middle-aged and elderly. There was any significant difference between the levels of HSP70 (P=0.741), MDA (P=0.539), PC (P=0.075) and GPx (P=0.071) liver tissue in different ages (young, middle-aged and elderly) with pre-treatment and aerobic exercise. Conclusion: Our study suggests that liver protection induced by chronically exercise in DOX treated rats is associated with inhibition of oxidative stress and increase in the efficiency of antioxidant system.
