One of the crucial parameters in the electrospinning process is temperature, which not only affects the quality of the oil/water separation, but also can change the morphology and porosity of nanofibers. In this work, the polyacrylonitrile (PAN) membrane was fabricated at two different electrospinning temperatures of 25 and 35 °C. PAN membranes were characterized by crosssectional Field Emission Scanning Electron Microscopy FESEM, water contact angle (WCA) and underwater oil contact angle (UOCA) analysis. The porosity, water flux, oil/water emulsion and oil rejection of the both prepared PAN membranes were also investigated