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Shadmehr Mirdar Harijani

Shadmehr Mirdar Harijani

Academic rank: Professor
Education: PhD.
Faculty: Faculty of Physical Education and Sports Sciences
Address: Professor of Exercise Physiology, Dep. of Exercise physiology, Faculty of sports Sciences, University of Mazandaran P.O.Box 47415-388 Babolsar,Mazandaran Province, IRAN Tel: +98 +111+35302201 & 35302254 Fax: +98+ 111+35302201
Phone: 01135302254


Measurement of levels of lung HIF-1α protein in response to tapering for 14- and 21-day with nigella sativa supplementation in maturing rat, with histological study
Volume taper, HIF-1a , Nigella sativa ,Lung parenchyma, Lung histology
Journal Sport Sciences for Health
Researchers Ehsan Arab zadeh ، Shadmehr Mirdar Harijani ، zohre fathi bayatiani


The aim of this study was to evaluate lung histology and the levels of HIF-1a protein in response to the reduction in training volume for 14 and 21 days with Nigella sativa (NS) supplementation. 60 three-week-old male Wistar rats (68 ± 9 g) were divided into 6 groups after 6 weeks of incremental interval training (IIT); IIT control and IIT groups, 2 groups of 14-day taper (with and without NS) and, similarly, 2 groups of 21-day taper. After 6 weeks, NS seed ethanol extract was fed daily by gavages to NS groups at 500 mg/kg. Finally, animals were euthanized and their lungs were removed, weighed and fixed in 10 % formalin. By routine and standard paraffin embedding, lung samples were sectioned in 5 lm and stained with H&E and studied histologically. The level of HIF-1a was measured by ELISA kit. The results showed that the administration of NS and 21-day taper in the IIT exposed rats caused significant difference in lung weight and lung HIF-1a (p = 0.001, p = 0.001). But the interaction between groups and taper periods was not confirmed (p = 0.530). Lung histology in 21-day taper with NS was better than the other groups. The results showed that after IIT, 21-day reduction in training volume and the use of new strategies such as NS supplementation in this period can reduce HIF-1a higher amounts in the lungs, which may probably be due to the anti-inflammatory effects of the NS supplement. These agents can improve respiratory capacity for competition or severe activity after taper period.