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Sakineh Asghari

Sakineh Asghari

Academic rank: Professor
ORCID:
Education: PhD.
ScopusId:
HIndex:
Faculty: Faculty of Chemistry
Address: Department of Organic Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran
Phone: 01135302357

Research

Title
Determination of vitamin B6 using an optimized novel TCPO–indolizine–H2O2 chemiluminescence system
Type
JournalPaper
Keywords
peroxyoxalate, hydrogen peroxide, chemiluminescence, indolizine derivatives, vitamin B6
Year
2014
Journal LUMINESCENCE
DOI
Researchers Mohammad Javad Chaichi ، mahjoobeh ehsani ، Sakineh Asghari ، Vahideh Behboodi

Abstract

Indolizine derivatives are of great interest as fluorescent emitters for peroxyoxalate chemiluminescence. The reaction of peroxyoxalates such as bis-(2,4,6-trichlorophenyl) oxalate (TCPO) with H2O2 can transfer energy to fluorescer via the formation of dioxetanedione intermediate. Four indolizine derivatives were used as a novel fluorescer in the chemiluminescence (CL) systems in this study. The relationship between CL intensity and the concentration of fluorescer, peroxyoxalate, sodium salicylate and hydrogen peroxide was investigated. Optimum conditions were obtained for four fluorescers and it was found that the indolizine can be used as an efficient green fluorescence emitter. Vitamin B6 induces a sharp decrease in the CL intensity of the TCPO–hydrogen peroxide–sodiumsalicylate system. A simple, rapid and sensitive CL method for the determination of vitamin B6 has been developed. The results showed a linear relationship between vitamin B6 concentration and peroxyoxalate CL intensity in the range 7.0× 108–1.0× 104. A detection limit of 2.3× 108 M and relative standard deviation (RSD) of <4.5% were obtained.