This research consisted of the following steps: (a) synthesis of new organic modifier, (b) preparation of novel generation of silane coupling agent, (c) fabrication of organic modified silica NPs, and (d) preparation of polyvinyl alcohol-based NCs. The obtained materials were characterized by different spectroscopic methods. Also, the microbial activities of surface-modified silica bionanoparticles on Grampositive and Gram-negative bacteria were investigated.