Lead-203 was produced by proton bombardment of natural thallium target. For producing 203Pb, natTl was electroplated on copper backing as target and was bombarded by energetic proton beam. After bombardment, the target was dissolved in 1 M HNO3. Ion exchange, solvent extraction, and nanomagnetic ion-imprinted polymer methods were used for separating 203Pb from thallium. The separation efficiency of these methods was studied. γ-Ray spectroscopy was applied for evaluating 203Pb and others radionuclides. The separation efficiency was determined from the γ-ray spectra. The separation efficiency of the methods using ion exchange, solvent extraction, and nanomagnetic ion-imprinted adsorbent was 98, 99.3, and 96.4%, respectively.