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Fatemeh Roodbari

Fatemeh Roodbari

Academic rank: Assistant Professor
ORCID:
Education: PhD.
ScopusId:
Faculty: Science
Address: University of Mazandaran
Phone: 01135302424

Research

Title
Prevalence and genetic diversity of norovirus genogroup II in children less than 5 years of age with acute gastroenteritis in Tehran, Iran
Type
JournalPaper
Keywords
Norovirus Gastroenteritis Genotyping RdRp Capsid
Year
2018
Journal MEDICAL MICROBIOLOGY AND IMMUNOLOGY
DOI
Researchers Mahsa Farsi ، Fatemeh Roodbari ، Behrooz Nejati ، Arash Arashkia ، Somayeh Jalilvand ، Zabihollah Shoja ، Alireza Nateghian ، Aliakbar Rahbarimanesh

Abstract

Viral gastroenteritis is a major public health problem worldwide. In Iran, very limited studies have been performed with regard to the epidemiology of noroviruses. This study aimed to evaluate the prevalence and molecular epidemiology of GII noroviruses in hospitalized children less than 5 years of age with acute gastroenteritis (AGE). A total of 210 stool specimens were collected from Ali Asghar Children’s Hospital and Bahrami Children’s Hospital in Tehran, from June 2015 to June 2016. The samples were screened by real-time RT-PCR for genogroup II (GII). Positive samples were genotyped by semi-nested PCR followed by Sanger sequencing and phylogenetic analysis. Norovirus was identified in 36 (17.1%) of 210 specimens. Based on genetic analysis of RdRp and capsid sequences, the strains were clustered into eight RdRp–capsid genotypes: GII.P4–GII.4 Sydney_2012 (41.7%), GII.Pe–GII.4 Sydney_2012 (30.6%), GII.P21–GII.3 (13.9%), GII.P16–GII.4 Sydney_2012 (2.8%), GII.P16–GII.12 (2.8%), GII.P2–GII.4 Sydney_2012 (2.8%), GII.P7–GII.7 (2.8%) and GII.P2–GII.2 (2.8%). We determined several different co-circulating norovirus genotypes in children < 5 years of age with AGE in our hospital in Tehran, Iran. Continued molecular surveillance of noroviruses, including typing of both RdRp and capsid genes, is important for monitoring emerging strains in our continued efforts to reduce the overall burden of norovirus disease.