Staphylococcus aureus is the most important human pathogen of Staphylococcus genus. S. aureus can cause a wide range of illnesses from skin infections to dangerous diseases, such as endocarditis, osteomyelitis, pneumonia, etc. This bacteria that is a main cause of hospitaland and community-acquired infections, due to high damage and increasing resistance against a variety of antibacterial drugs, has become one of the major public health concerns. Increasing rate of methicillin resistant Staphylococcus aureus (MRSA) and vancomycin-resistant strains (VRSA), made the treatment of staphylococcal infections difficult. The aim of this study is to evaluate the prevalence of vancomycin-resistant strains among isolates of methicillin-resistant Staphylococcus aureus clinical samples.