ABSTRACT BACKGROUND AND OBJECTIVES Nanobiotechnology advancements have resulted in intriguing discoveries in science. Biosynthesis of economically and environmentally beneficial metal nanoparticles can now be done using this technology in the fields of agriculture, medicine and etc. plant components including leaves, roots, and fruits are used for biosynthesis applications, also known as “green synthesis”. MATERIALS AND METHODS Herein we report the green synthesis of silver nanoparticles (AgNPs) completed using garlic (Allium sativum) leaf extract. Characterization of nanoparticles was carried out by UV-Vis spectroscopy, FESEM, MAP and EDS analysis. Antimicrobial activity of the AgNPs was evaluated against five distinct bacterial strains, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 13883 and Proteus mirabilis ATCC 43071, using well and disc diffusion method. RESULTS AND DISCUSSION The UV-Vis spectroscopy showed an absorbance peak range between 390nm to 500nm which indicates the presence of AgNPs in the reaction solution. Using FESEM the size of the AgNPs was found to be remarkably small with a size range of 15nm to 17 nm, and spherical. The biosynthesized AgNPs also showed promising antimicrobial activity against the bacterial strains, S. aureus showed the highest sensitivity with an inhibitory zone around 14mm whereas the gram-negative strains showed less sensitivity towards the biosynthesized AgNPs. CONCLUSION In this study the aerial leaves of Allium sativum were used, since they are considered agricultural waste so we could provide a way to turn them into profit, in doing so biosynthesis of stable spherical AgNPs was demonstrated using A. sativum leaf extract, where it acts as a reducing agent and was able to biosynthesize AgNPs with a high antibacterial activity which can have applications in industries and medicine.
