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Fatemeh Roodbari

Fatemeh Roodbari

Academic rank: Assistant Professor
ORCID:
Education: PhD.
ScopusId:
Faculty: Science
Address: University of Mazandaran
Phone: 01135302424

Research

Title
Cellular and Humoral Immune Responses against AS03-Inactivated SARS CoV-2 Vaccine
Type
Presentation
Keywords
inactivated vaccine, covid19, AS03, IFN-γ, IL-4
Year
2023
Researchers Mahla Abbasi ، Fatemeh Roodbari ، Akbar Khorasani ، Mehdi Mahdavi

Abstract

Background: Several vaccines in the world are commercialized for COVID-19 infection. One of these vaccines is inactivated SARS-CoV-2 vaccine which is formulated in Alum adjuvant that is not capable of the induction of cellular immune responses. In the present study, inactivated SARS CoV-2 virus was formulated in AS03 adjuvant and the immunogenicity was compared with the Alum-based vaccine. Methods: Experimental 6-8-week-old BALB/c mice were immunized three times at three weeks intervals with AS03- and Alum-based vaccines along with PBS as a control group. Lymphocyte proliferation of spleen cells was performed by the BrdU method, IL-4 and IFN-γ cytokines were assessed on the spleen cell culture supernatant by quantitative ELISA kits. Specific total IgG and IgG1/IgG2a were assessed with an optimized indirect ELISA. Results: The results showed that the vaccine formulated in AS03 adjuvant led to a significant lymphocyte proliferation response versus Alum-based and PBS control group. Conclusion: In addition, the cytokines responses showed a Th1 platform in our vaccine formulation. Furthermore, IgG response showed an improvement versus the other experimental groups.