2-chlorobenzoyl ferrocene, was synthesized and used to construct a modified carbon nanotube paste electrode. The electrooxidation of N-acetylcysteine at the surface of the modified electrode was studied. Under the optimized conditions, the square wave voltammetric (SWV) peak current of Nacetylcysteine increased linearly with N-acetylcysteine concentration in the ranges of 5.0 ×10−8 to 4.0 ×10−4 M. The prepared modified electrode exhibits a very good resolution between the voltammetric peaks of N-acetylcysteine and folic acid which makes it suitable for the detection of N-acetylcysteine in the presence of folic acid in real samples.