Glucoraphanin is a kind of aliphatic glucosinolates found almost exclusively in broccoli. Glucoraphanin is converted into sulphoraphane by the beneficial bacteria that live in human digestive systems. Many studies around the world have been documented broccoli with reducing the risks of developing certain chronic diseases such as cancer and heart disease as well as having antioxidant defences [1]. Glucosinolates are biosynthesized from amino acids. Aliphatic glucosinolates are mostly derived from methionine. The biosynthesis of aliphatic glucosinolates starts with chain elongation of methionine which performs by MAM enzymes (MAM1 and MAM3). Then the biosynthesis is continued with formation of a core glucosinolate structure, and secondary modification [2]. In silico analysis, using diverse biological databases such as protein-protein interaction, co-localization, shared protein domain and co-expression, facilitates functional analysis and provides valuable insight about biological relationship among genes/proteins [3]. Our in silico analysis by GeneMANIA algorithm [4] displayed protein-protein interaction between F22O13_24 and two other proteins (MAM1 and MAM3).Since both MAM1 and MAM3 are involved in aliphatic glucosinolate biosynthesis, therefore, F22O13_24 may have a role in biosynthesis of glucoraphanin.
