Glucosinolates (a large group of sulfur-containing secondary plant metabolites) are responsible for medicinal property of all economically important Brassicaceae as well as the model plant Arabidopsis thaliana. In recent years, glucosinolates and their breakdown products have been the subject of extensive studies due to their role in human [1]. It has been studied the beneficial impact of some glucosinolates and their breakdown products on carcinogenesis, cardiovascular and neurological diseases [2]. Based on the precursor amino acid used, glucosinolates are classified into three major groups namely aliphatic, indolic and aromatic [1]. The glucosinolate content and composition may be affected by pathogen attack, nutrient supply, especially sulfur and nitrogen [3], but also by environmental factors such as temperature, length of the photoperiod, season, drought and salinity. During biotic and abiotic stresses, glucosinolate biosynthesis is further controlled by a complex network of transcription factors. Our previous investigation, proteomic data and in silico analysis, suggested that AT3G47570 gene may display a role in glucosinolate biosynthesis pathway. Indeed, the possible role of AT3G47570 in plant defence [4] and nitrogen starvation response [5] have already been documented. In silico analysis, using diverse biological databases facilitates functional analysis, directs lab experiments and provides valuable insight about biological relationship among genes/proteins. These biological databases have been embedded in GeneMANIA algorithm, such as protein-protein interaction, co-localization, gene-gene interaction and co-expression revealed biological connections between AT3G47570 and glucosinolate biosynthesis pathway genes. AT3G47570 gene is co-expressed with SOT16, CYP83B1 and MYB28. It has been also co-localized with SUR1 and SOT17. In silico analysis suggested that AT3G47570 gene as a candidate gene which probably regulate glucosinolate biosynthesis pathway.
